Avidin is a tetrameric protein commonly isolated from egg whites. Avidin binds to biotin with strength approaching that of a covalent bond. Disruption of the avidin-biotin bond occurs only under extraordinary conditions, such as pH 1.5 with 6 M guanidium hydrocholoride.
Fluorescent avidin can bind to biotinylated antibodies used in immunohistochemistry and immunocytochemistry. Avidin thiol can bind to gold surfaces and act as a glue for biotinylated proteins. Avidin agarose is useful for efficient removal of biotinylated proteins from a liquid phase.
Avidin
(sulfhydryl)
product code: SVT
from egg white. 10-14 units/mg of solid.
product code: AVN
More Details
1 unit equals the protein required to bind 1 microgram of d-biotin at pH8.9
(sulfhydryl)
product code: AVT
LI-COR compatible
(700 nm) channel
product code: AVY
TMR (tetramethylrhodamine)
product code: AVR
cross-linked agarose beads
product code: AVG
(sulfhydryl)
product code: NVT